ABSTRACT
Objective: The present work was undertaken with an aim to develop and validate a rapid reverse-phase high-performance liquid chromatography (RP-HPLC) method for the estimation of curcumin and cyclosporine in the capsule dosage form. Methods: The RP-HPLC method for the simultaneous estimation of curcumin and cyclosporine was developed using Agilent (Infinity 1260) HPLC system and Eclipse XDB-C18 (4.6 x 150 mm i.d., 5µ) stationary phase. The optimized mobile phase comprised of acetonitrile: water: methanol (50: 10: 40 v/v/v) pumped at a flow rate of 0.5 ml/min. Separation of drugs was achieved in an isocratic mode and elution was monitored using PDA detector at 214 nm. The method was validated as per ICH-Q2R1 guidelines. Results: Retention time of the curcumin and cyclosporine were found to be 3.073 min and 6.373 min with the correlation coefficient (R2) of 0.9993 and 0.998 respectively. The response of curcumin and cyclosporine was found linear in the concentration range of 8-48 μg/ml and 4-24 μg/ml respectively. The percent recovery values were found in the range of 97-103% indicating satisfactory accuracy of the method. The percent relative standard deviation (% RSD) values for the precision study was less than 2 which suggest that the method is precise. Conclusion: The proposed method was found accurate, precise and specific for the determination of curcumin and cyclosporine in bulk as well as in capsule dosage form. Thus, the present method can be used for routine analysis and quality control of curcumin and cyclosporine in bulk and capsule dosage form.
ABSTRACT
Aim: To investigate the antioxidant and antimicrobial potential of Chloroform and Pet ether extracts of Manilkara zapota (MZCE, MZPE), Polyalthia longifolia (PLCE, PLPE), Abroma augusta (AACE, AAPE) Ficus hispida (FHCE, FHPE), Vitex negundo (VNCE, VNPE) plants. Study Design: In vitro antioxidant and antimicrobial study. Place and Duration of Study: Department of Pharmacy, School of Science & Engineering, Southeast University, Banani, Dhaka between June 2011 and March 2012. Methodology: In vitro antioxidant activity was performed using DPPH radical scavenging, nitric oxide (NO) scavenging, reducing power, total antioxidant capacity, total phenol and total flavonoid content determination assays. The antimicrobial assay was performed by disc diffusion method using kanamycin and Nystatin as the standard. Results: The most prominent antioxidant activity was observed with PLPE in DPPH radical scavenging test (IC50 =191.308 ± 28.450 μg/ml) as opposed to that of standard ascorbic acid (IC50= 43.129 ± 1.181μg/ml). In total antioxidant capacity method, FHCE showed the highest activity (837.558 ± 110.835 mg ascorbic acid/g). The total phenolic and flavonoids content were determined by Folin–Ciocalteu Reagent and aluminum chloride colorimetric method respectively. The highest total phenols & total flavonoids content were found in VNPE (180.434 ± 142.19 mg Gallic acid/g & 1265.255 ± 165.593 mg quercetin/g, respectively). The ferric reducing capacity of the extracts was strong and dose dependent manner. PLPE displayed the highest antimicrobial actions against Bacillus megaterium (40 mm). Conclusion: Comparison of different plant extracts used in the present study in various tested models showed wide variations in phenolic content and varying degrees of radical scavenging & reducing capacity. The obtained results indicate that investigated plants could be potential sources of natural antioxidants & antimicrobial agents and can be used for infectious diseases.